Fiber-optic detection of pH for in-vivo analysis of sub-microliter natural samples

Year: 2013

Ondřej Podrazký, Ph.D.

Cooperation: Institute of Experimental Botany AS CR, Safibra s.r.o.

We developed miniature fiber optic pH-meter based on ratiometric fluorescence principle. Device can determine pH in a range of 5.5-7.5 with a resolution of 0.1 during several seconds. The probe allows the minimally invasive monitoring of natural living samples (tissues, embryos, body liquids) to be performed and can even be used for in-vivo measurements in living cells. Results important for plant nutrition and agriculture production were achieved by measurement of hair roots of Arabidopsis th.

Fiber optic detection of pH in Arabidopsis thaliana


The scheme of measurement system, the photography of the sample space with a micromanipulator and the microscope photography of a probe inside the root of Arabidopsis thaliana.


  1. Kašík, Ivan ; Podrazký, Ondřej ; Mrázek, Jan ; Martan, Tomáš ; Matějec, Vlastimil ; Hoyerová, Klára ; Kamínek, Miroslav. In vivo optical detection of pH in microscopic tissue samples of Arabidopsis thaliana. .i.Materials Science & Engineering C-Biomimetic and Supramolecular Systems./i., 2013

IPE carries out fundamental and applied research in the scientific fields of photonics, optoelectronics and electronics. In these fields, IPE generates new knowledge and develops new technologies.

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