Amyloid ß peptides and mitochondrial enzyme 17ß-hydroxysteroid dehydrogenase type 10, possibility of Alzheimer disease diagnostics using optical biosensors

Levels of enzyme 17ß-hydroxysteroid dehydrogenase type 10 and of its complex with Aß will be estimated by means of different methods (ELISA, Western blot, biosensors) in CSF of patients with Alzheimer disease and nondemented controls. The first aim is toevaluate new perspective bioindicators of Alzheimer disease that could be used in clinical diagnosis. The second aim is to evaluate alterations in the expression of HSD-10 in the brains of male Wistar and Long Evans rats occurring during normal aging.