In this project, I introduce the next generation of super-resolution microscopy relying on direct and label-free imaging of single-biomolecule fluctuations at high-speed to offer a complementary insight into the complex dynamics of living systems. The proposed method is based on the detection and three-dimensional localization of all (resolvable) fluctuations associated with interacting single biomolecules. I leverage interferometric scattering microscopy (iSCAT) to detect the light scattered by a single molecule and achieve microsecond temporal resolution to discern molecule-by-molecule details of protein assemblies and turnover in large biomolecular structures. I aim to enhance the experimental temporal resolution to capture discrete molecular interactions, such as binding, unbinding, or displacement events, within each frame, ensuring their localization remains sparser than the light’s diffraction limit.
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