The project is aimed at detail investigation of structural arrangement, formation of biologically relevant complexes, and dynamics of selected representative types of nucleic acid regulatory sequences – DNA guanine quadruplexes, miRNA sequences, and DNA transcription elements containing CArG box – in order to elucidate the molecular mechanism of their regulatory function and its control by external factors, namely molecular crowding, temperatures and charge compensating molecular surrounding. The nucleic acid segments and/or their complexes with key proteins will be studied by means of SPR biosensing, Raman and NMR spectroscopy, CD spectroscopy, Raman optical activity, and molecular dynamics simulations. These methods will be optimized and the experimental and theoretical approaches further developed for advanced investigation of nucleic acids.
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