The project will deal with properties of important nucleic acids (NA) segments from the viewpoint of their structural fluctuations and formation or decay kinetics of structural motifs and/or their complexes. Main accent will be put on employment of a large set of advanced experimental techniques including micro-Raman spectroscopy, sensors based on surface plasmon resonance (SPR), and dynamic NMR measurements on 13C-labeled nucleotides, complemented by molecular dynamics simulations. Investigation will be concentrated to several families of NA structural motifs significant for natural and/or artificial regulation of gene expression. Besides deep understanding of structural and dynamic properties of the natural and chemically modified NA motifs, important development and improvement of certain experimental techniques will be achieved, in particular development of mixing microfluidic system for kinetic micro-Raman measurements and implementation of a new concept of SPR imaging with polarization contrastfor highly parallelized measurements.
Menu